Research Article
Comparison of elastic tissue stain with haematoxylin and eosin stain in the detection of venous invasion in colorectal carcinoma in the Sri Lankan setting
Authors:
P. N. Yogananda ,
National Hospital of Sri Lanka, LK
About P. N.
Department of Pathology
S. R. Constantine,
National Hospital of Sri Lanka, LK
About S. R.
Department of Pathology
K. Rajeewan,
National Hospital of Sri Lanka, LK
About K.
Department of Pathology
W. R. A. T. J. Ranasinghe
Base Hospital Homagama, LK
About W. R. A. T. J.
Department of Pathology
Abstract
Introduction and objective: Colorectal carcinoma (CRC) is the third leading cause of cancer among both males and females in Sri Lanka. Venous invasion (VI) by the tumour is a well-recognized independent prognostic factor in CRC and a key high-risk factor that determines the need for adjuvant therapy in stage II CRC. It is widely accepted that VI is under-recognised on haematoxylin and eosin (H&E)-stained tissue sections of the CRC resection specimens. The main objective of this study was to evaluate whether the VI detection rate on an elastic tissue stain (ETS) is superior to that of the VI detection rates on a routine H&E stain in CRC resection specimens in a Sri Lankan setting.
Methodology: This was a retrospective, descriptive study with an analytical component carried out at the Department of Histopathology, National Hospital of Sri Lanka. Data were retrieved retrospectively from 386 cases of CRC. The cases with no VI on the H&E stain were subjected to ETS and re-examined for the presence of VI on both H&E and ETS. The results of the two stains were compared statistically using the chi-square test.
Results: VI was present in 152 of the 386 specimens (39.38%) on the initial H&E-stained sections. Of the remaining 234 specimens, ETS enabled the detection of VI in a further 122 cases, increasing the overall VI detection rate to 70.98% of CRC resection specimens (X2=102.490, P<0.001).
Conclusion: The addition of an ETS enhances the histological detection of VI in CRC resection specimens.
How to Cite:
Yogananda, P.N., Constantine, S.R., Rajeewan, K. and Ranasinghe, W.R.A.T.J., 2022. Comparison of elastic tissue stain with haematoxylin and eosin stain in the detection of venous invasion in colorectal carcinoma in the Sri Lankan setting. Journal of Diagnostic Pathology, 17(1), pp.1–10. DOI: http://doi.org/10.4038/jdp.v17i1.7820
Published on
31 Dec 2022.
Peer Reviewed
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