Start Submission Become a Reviewer

Reading: Assessment of the impact of the concentration of formalin on receptor studies in surgical sp...

Download

A- A+
dyslexia friendly

Articles

Assessment of the impact of the concentration of formalin on receptor studies in surgical specimens of breast carcinoma: a pilot study

Authors:

M B S Senevirathne ,

University of Sri Jayewardenepura, Gangodawila, Nugegoda, LK
About M B S
Department of Pathology, Faculty of Medical Sciences
X close

D H Beneragama

University of Sri Jayewardenepura, Gangodawila, Nugegoda, LK
About D H
Department of Pathology, Faculty of Medical Sciences
X close

Abstract

Introduction: Assessment of oestrogen, progesterone and HER2 receptor status are routinely performed on specimens fixed in 10% formalin.

Objective: To assess the impact of the concentration of formalin on the oestrogen, progesterone & HER2 receptor status in surgical specimens excised for carcinoma of breast.

Method: A descriptive, cross sectional study was done on excision biopsy and mastectomy specimens of breast cancer over a period of 18 months. A 1x1cm piece of tumour tissue was fixed in 50% diluted formalin and the remainder in 10% formalin. Following routine tissue processing, staining for ER, PR and HER2 receptor status was done. Allred scoring system was used to interpret ER and PR receptor status and CAP (College of American Pathologists) guidelines were used to interpret HER2 receptor status.

Results: In view of the small sample size (n=20), binomial sign test was selected to assess the differences between the two groups (10% formalin vs 50% diluted formalin). There were statistically significant differences in the results with a marked downgrading of the total score of ER and PR and HER 2 receptor studies with 50% diluted formalin.

Conclusion: It is vital for the specimen to be adequately fixed in 10% buffered formalin before commencing on receptor studies.

DOI: http://dx.doi.org/10.4038/jdp.v9i1.7651

Journal of Diagnostic Pathology 2014; 9(1): 24-32

How to Cite: Senevirathne, M.B.S. & Beneragama, D.H., (2014). Assessment of the impact of the concentration of formalin on receptor studies in surgical specimens of breast carcinoma: a pilot study. Journal of Diagnostic Pathology. 9(1), pp.24–32. DOI: http://doi.org/10.4038/jdp.v9i1.7651
Published on 20 Nov 2014.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus